Gene Editing Lab Technician (2nd Shift) South San Francisco
Ohalo Genetics, Inc, San Francisco, CA, United States
Position
Gene Editing Lab Technician (2nd Shift)
Schedule
Shift: 2nd Shift (3pm - 11pm)
Type
Full-time
Opportunity
Ohalo Genetics is seeking a motivated Gene Editing Lab Technician who will contribute to the advancement of plant variety development. The successful candidate will be detail-oriented, enthusiastic about hands‑on work, and responsible. Prior experience in a sterile work environment, plant breeding or gene editing is a plus, but is not required. The Gene Editing Lab Technician will assist in specific duties to ensure the overall success of the gene editing laboratory. This role will involve additional responsibilities related to lab operations, plant inventory maintenance, and process development. This role is not limited to only lab work, and offers opportunities to contribute to various aspects of our lab's growth.
Responsibilities
Protoplast Isolation, Transfection, and Culture:
Perform enzymatic digestion of plant tissues (e.g., leaf, root, or callus) to isolate viable protoplasts.
Transfect protoplasts using PEG‑mediated transfection or electroporation methods.
Culture transfected protoplasts to regenerate callus.
Contribute to the continuous improvement and documentation of SOPs for protoplast workflows.
Microscopy and Imaging:
Assess protoplast yield, viability, and cell health using fluorescence and confocal microscopy and viability dyes (e.g., FDA staining).
Data Recording and Analysis:
Maintain accurate and detailed electronic lab notebooks (ELN) and batch records.
Compile and analyze experimental data using tools such as ImageJ, GraphPad Prism, or Excel.
Sample Preparation:
Prepare samples for downstream molecular analyses, including RNA extraction, protein extraction, and genomic DNA isolation.
Cross‑Functional Support and Collaboration:
Collaborate with other Technicians, Research Associates, and Scientists on workflows, ensuring that critical processes are completed.
Lab Maintenance and Compliance:
Follow safety protocols and institutional guidelines for working with plant tissue and enzymes.
Perform all tasks in a manner consistent with and in support of all company policies and practices related to safety and compliance.
Perform other duties as assigned.
Candidate Profile
Ability to accurately follow verbal and written instructions and suggest optimizations to methodologies.
Ability to sit for extended periods and perform repetitive motions.
Proactive approach to routine tasks.
Passion for learning and enthusiasm for plants and hands‑on work.
Positive attitude and strong interpersonal skills.
Ability to ask clarifying questions.
Work independently and in a team environment across multiple departments.
Must be able to lift up to 50 pounds on an intermittent basis and sit for extended periods.
Prioritize safety in all activities.
Desired Skills/Experience
Experience working in a lab including sterile technique, micropropagation, media preparation, microscopy, and DNA extraction.
Compensation
Anticipated Compensation: $33/hr
Equal Opportunity Statement
Ohalo is an Equal Opportunity/Affirmative Action employer committed to diversity in the workplace. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, age, national origin, disability, protected veteran status, gender identity or any other factor protected by applicable federal, state or local laws. Ohalo is also committed to working with and providing reasonable accommodations to individuals with disabilities. Please let your recruiter know if you need an accommodation at any point during the interview process.
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Gene Editing Lab Technician (2nd Shift)
Schedule
Shift: 2nd Shift (3pm - 11pm)
Type
Full-time
Opportunity
Ohalo Genetics is seeking a motivated Gene Editing Lab Technician who will contribute to the advancement of plant variety development. The successful candidate will be detail-oriented, enthusiastic about hands‑on work, and responsible. Prior experience in a sterile work environment, plant breeding or gene editing is a plus, but is not required. The Gene Editing Lab Technician will assist in specific duties to ensure the overall success of the gene editing laboratory. This role will involve additional responsibilities related to lab operations, plant inventory maintenance, and process development. This role is not limited to only lab work, and offers opportunities to contribute to various aspects of our lab's growth.
Responsibilities
Protoplast Isolation, Transfection, and Culture:
Perform enzymatic digestion of plant tissues (e.g., leaf, root, or callus) to isolate viable protoplasts.
Transfect protoplasts using PEG‑mediated transfection or electroporation methods.
Culture transfected protoplasts to regenerate callus.
Contribute to the continuous improvement and documentation of SOPs for protoplast workflows.
Microscopy and Imaging:
Assess protoplast yield, viability, and cell health using fluorescence and confocal microscopy and viability dyes (e.g., FDA staining).
Data Recording and Analysis:
Maintain accurate and detailed electronic lab notebooks (ELN) and batch records.
Compile and analyze experimental data using tools such as ImageJ, GraphPad Prism, or Excel.
Sample Preparation:
Prepare samples for downstream molecular analyses, including RNA extraction, protein extraction, and genomic DNA isolation.
Cross‑Functional Support and Collaboration:
Collaborate with other Technicians, Research Associates, and Scientists on workflows, ensuring that critical processes are completed.
Lab Maintenance and Compliance:
Follow safety protocols and institutional guidelines for working with plant tissue and enzymes.
Perform all tasks in a manner consistent with and in support of all company policies and practices related to safety and compliance.
Perform other duties as assigned.
Candidate Profile
Ability to accurately follow verbal and written instructions and suggest optimizations to methodologies.
Ability to sit for extended periods and perform repetitive motions.
Proactive approach to routine tasks.
Passion for learning and enthusiasm for plants and hands‑on work.
Positive attitude and strong interpersonal skills.
Ability to ask clarifying questions.
Work independently and in a team environment across multiple departments.
Must be able to lift up to 50 pounds on an intermittent basis and sit for extended periods.
Prioritize safety in all activities.
Desired Skills/Experience
Experience working in a lab including sterile technique, micropropagation, media preparation, microscopy, and DNA extraction.
Compensation
Anticipated Compensation: $33/hr
Equal Opportunity Statement
Ohalo is an Equal Opportunity/Affirmative Action employer committed to diversity in the workplace. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, age, national origin, disability, protected veteran status, gender identity or any other factor protected by applicable federal, state or local laws. Ohalo is also committed to working with and providing reasonable accommodations to individuals with disabilities. Please let your recruiter know if you need an accommodation at any point during the interview process.
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