Research Technician
University of South Florida, Tampa, FL, United States
Organizational Summary
The USF Health Taneja College of Pharmacy is managed by the Dean and an actively engaged administrative leadership team. The mission of the college is to transform health through educating students to be outstanding and successful practitioners and leaders in the profession, through leading in discovery and innovation of education and practice in pharmacy, and through delivering the highest quality care in diverse, interprofessional clinical and community settings.
Job Summary
Applicant will be assigned to work on research areas of heart diseases in Dr. Tipparaju lab at the Department of Pharmaceutical Sciences. Dr. Tipparaju’s research lab studies heart failure, myocardial infarction, skeletal muscle disease and evaluates gene/drug
anoparticle‑based therapeutics using cell culture, organoids and animal models.
Responsibilities
Conduct research using ex vivo Langendorff setup, performing I/R protocols, collecting perfusate, measuring biochemical markers, TTC staining, and compiling timely reports using Image J, Biorender, GraphPad Prism.
Develop and modify laboratory research protocols on biological materials, including mammalian tissue cultures, histology with sterile technique, slide preparation and staining, and assays for protein (Western blotting), RNA, DNA, or other cellular components.
Care for laboratory animals, performing standardized experimental protocols and in vivo procedures, preparing animals for surgery, and caring for animals after surgery, including coordination of animal handling, breeding, tissue collection, and genotyping.
Culture and differentiate induced pluripotent stem (iPS) cells.
Maintain laboratory equipment, arrange repairs, order and inventory supplies, calibrate instruments, and prepare equipment for use.
Keep records and maintain project databases, prepare graphs, charts, and tables from experimental results for presentations, publications, and grant applications.
Train undergraduate students in laboratory methods, perform literature searches for appropriate laboratory techniques, and perform other duties as assigned.
Be available on weekends if needed to change culture medium for stem cells and care for animals.
Attend lab meetings and present data to PI and lab members.
Minimum Qualifications
High school diploma or equivalent plus three years of laboratory or research experience; college coursework in a natural or physical science may substitute for experience on a year‑to‑year basis.
Four years of direct experience for a bachelor’s degree (SB 1310 equivalency).
Eligibility for federal employment (meeting all U.S. work‑authorization requirements).
Preferred Qualifications
Bachelor’s degree in biology, chemistry, health sciences, pharmaceutical, medical, or applied science.
Experience with immunohistochemistry, confocal microscopy, western blotting, real‑time qPCR, and related techniques.
Experience in cell culture research is a plus.
Equal Employment Opportunity
The University of South Florida is an equal opportunity employer that does not discriminate against any employee or applicant for employment based on any characteristic protected by law. The University maintains affirmative action programs for protected veterans and individuals with disabilities in accordance with all applicable federal and state laws. This job description does not constitute an employment contract.
Background Check
This position may be subject to a Level 1 or Level 2 criminal background check. Applicants have rights under federal employment laws.
#J-18808-Ljbffr
The USF Health Taneja College of Pharmacy is managed by the Dean and an actively engaged administrative leadership team. The mission of the college is to transform health through educating students to be outstanding and successful practitioners and leaders in the profession, through leading in discovery and innovation of education and practice in pharmacy, and through delivering the highest quality care in diverse, interprofessional clinical and community settings.
Job Summary
Applicant will be assigned to work on research areas of heart diseases in Dr. Tipparaju lab at the Department of Pharmaceutical Sciences. Dr. Tipparaju’s research lab studies heart failure, myocardial infarction, skeletal muscle disease and evaluates gene/drug
anoparticle‑based therapeutics using cell culture, organoids and animal models.
Responsibilities
Conduct research using ex vivo Langendorff setup, performing I/R protocols, collecting perfusate, measuring biochemical markers, TTC staining, and compiling timely reports using Image J, Biorender, GraphPad Prism.
Develop and modify laboratory research protocols on biological materials, including mammalian tissue cultures, histology with sterile technique, slide preparation and staining, and assays for protein (Western blotting), RNA, DNA, or other cellular components.
Care for laboratory animals, performing standardized experimental protocols and in vivo procedures, preparing animals for surgery, and caring for animals after surgery, including coordination of animal handling, breeding, tissue collection, and genotyping.
Culture and differentiate induced pluripotent stem (iPS) cells.
Maintain laboratory equipment, arrange repairs, order and inventory supplies, calibrate instruments, and prepare equipment for use.
Keep records and maintain project databases, prepare graphs, charts, and tables from experimental results for presentations, publications, and grant applications.
Train undergraduate students in laboratory methods, perform literature searches for appropriate laboratory techniques, and perform other duties as assigned.
Be available on weekends if needed to change culture medium for stem cells and care for animals.
Attend lab meetings and present data to PI and lab members.
Minimum Qualifications
High school diploma or equivalent plus three years of laboratory or research experience; college coursework in a natural or physical science may substitute for experience on a year‑to‑year basis.
Four years of direct experience for a bachelor’s degree (SB 1310 equivalency).
Eligibility for federal employment (meeting all U.S. work‑authorization requirements).
Preferred Qualifications
Bachelor’s degree in biology, chemistry, health sciences, pharmaceutical, medical, or applied science.
Experience with immunohistochemistry, confocal microscopy, western blotting, real‑time qPCR, and related techniques.
Experience in cell culture research is a plus.
Equal Employment Opportunity
The University of South Florida is an equal opportunity employer that does not discriminate against any employee or applicant for employment based on any characteristic protected by law. The University maintains affirmative action programs for protected veterans and individuals with disabilities in accordance with all applicable federal and state laws. This job description does not constitute an employment contract.
Background Check
This position may be subject to a Level 1 or Level 2 criminal background check. Applicants have rights under federal employment laws.
#J-18808-Ljbffr