Research Assistant, Czyzyk-Krzeska Lab, College of Medicine, Department of Cance
Inside Higher Ed, Cincinnati, OH, United States
Job Overview
A Research Assistant position is available in the laboratory of Dr. M. Czyzyk-Krzeska in the Department of Cancer Biology at the University of Cincinnati College of Medicine. The laboratory investigates the molecular and metabolic mechanisms that drive renal cancer progression using advanced molecular biology, biochemistry, cell culture, and animal model systems. The Research Technician will support ongoing research by performing experimental procedures, maintaining cell cultures, assisting with animal studies, preparing reagents, organizing data, and contributing to the laboratory’s overall operation. The role offers hands‑on training in cutting‑edge cancer biology and direct mentorship from Dr. Czyzyk‑Kreczka and interaction with postdoctoral fellows, graduate students, clinical fellows, and undergraduate researchers.
Essential Functions
Perform experiments using standard wet‑lab molecular techniques and record data according to research protocols.
Develop and maintain protocols.
Interpret data and assist in judging data validity; may conduct statistical and graphical analysis.
Assist in animal colony management and husbandry.
Apply research principles to less complex assignments.
Perform related duties based on departmental need.
Required Education
Bachelor’s Degree. Four (4) years of relevant work experience and/or specialized training may substitute for the education requirement.
Required Experience
Additional related work experience may be required depending on the field or discipline.
Additional Qualifications Considered
DNA and RNA extraction and purification
PCR and quantitative real‑time PCR (qPCR)
Reverse transcription (cDNA synthesis)
Gel electrophoresis (agarose and polyacrylamide)
Western blotting and protein quantification (e.g., BCA assays)
Immunoprecipitation and co‑immunoprecipitation
Cloning, plasmid preparation, and bacterial transformation
Transfection and viral transduction (lentiviral/retroviral systems)
Cell culture maintenance, passaging, and viability assays
Flow cytometry sample preparation and basic analysis
Microscopy (bright‑field and fluorescence)
ELISA and other immunoassays
RNA interference (siRNA/shRNA knockdown)
Colony formation assays
Reporter gene assays (luciferase, GFP‑based assays)
Basic biochemistry techniques (protein extraction, purification, enzymatic assays)
Physical Requirements / Work Environment
Sitting – continuously
Repetitive hand motion – often
Hearing/listening – often
Talking – often
Standing – often
Walking – often
Bending – often
Stooping – often
Lifting (up to 20 lbs) – often
Kneeling, squatting – seldom
Noise – sufficient noise to require raising voice
Hazards – exposure to chemicals, moving machinery, high places, electrical current
Atmospheric conditions – fumes, odors, dust, mists, gases, poor ventilation
Compensation and Benefits
UC offers an exceptional benefits package designed to support well‑being, financial security, and work‑life balance.
Comprehensive Tuition Remission
– covers tuition costs for eligible students and dependents.
Robust Retirement Plans
– option between state pension plans (OPERS, STRS) or an Alternative Retirement Plan (ARP) with a 14–18% UC contribution.
Real Work‑Life Balance
– generous time‑off policy including vacation, sick time, 11 paid holidays, end‑of‑year paid time off, and six weeks paid parental leave.
Competitive salary
based on experience.
Comprehensive health coverage
(medical, dental, vision, prescription).
Flexible spending accounts & wellness programs .
Professional development & mentorship opportunities .
Equal Opportunity Employer
Building a workplace where all qualified applicants will receive consideration for employment, including Individuals with Disabilities and Protected Veterans.
#J-18808-Ljbffr
A Research Assistant position is available in the laboratory of Dr. M. Czyzyk-Krzeska in the Department of Cancer Biology at the University of Cincinnati College of Medicine. The laboratory investigates the molecular and metabolic mechanisms that drive renal cancer progression using advanced molecular biology, biochemistry, cell culture, and animal model systems. The Research Technician will support ongoing research by performing experimental procedures, maintaining cell cultures, assisting with animal studies, preparing reagents, organizing data, and contributing to the laboratory’s overall operation. The role offers hands‑on training in cutting‑edge cancer biology and direct mentorship from Dr. Czyzyk‑Kreczka and interaction with postdoctoral fellows, graduate students, clinical fellows, and undergraduate researchers.
Essential Functions
Perform experiments using standard wet‑lab molecular techniques and record data according to research protocols.
Develop and maintain protocols.
Interpret data and assist in judging data validity; may conduct statistical and graphical analysis.
Assist in animal colony management and husbandry.
Apply research principles to less complex assignments.
Perform related duties based on departmental need.
Required Education
Bachelor’s Degree. Four (4) years of relevant work experience and/or specialized training may substitute for the education requirement.
Required Experience
Additional related work experience may be required depending on the field or discipline.
Additional Qualifications Considered
DNA and RNA extraction and purification
PCR and quantitative real‑time PCR (qPCR)
Reverse transcription (cDNA synthesis)
Gel electrophoresis (agarose and polyacrylamide)
Western blotting and protein quantification (e.g., BCA assays)
Immunoprecipitation and co‑immunoprecipitation
Cloning, plasmid preparation, and bacterial transformation
Transfection and viral transduction (lentiviral/retroviral systems)
Cell culture maintenance, passaging, and viability assays
Flow cytometry sample preparation and basic analysis
Microscopy (bright‑field and fluorescence)
ELISA and other immunoassays
RNA interference (siRNA/shRNA knockdown)
Colony formation assays
Reporter gene assays (luciferase, GFP‑based assays)
Basic biochemistry techniques (protein extraction, purification, enzymatic assays)
Physical Requirements / Work Environment
Sitting – continuously
Repetitive hand motion – often
Hearing/listening – often
Talking – often
Standing – often
Walking – often
Bending – often
Stooping – often
Lifting (up to 20 lbs) – often
Kneeling, squatting – seldom
Noise – sufficient noise to require raising voice
Hazards – exposure to chemicals, moving machinery, high places, electrical current
Atmospheric conditions – fumes, odors, dust, mists, gases, poor ventilation
Compensation and Benefits
UC offers an exceptional benefits package designed to support well‑being, financial security, and work‑life balance.
Comprehensive Tuition Remission
– covers tuition costs for eligible students and dependents.
Robust Retirement Plans
– option between state pension plans (OPERS, STRS) or an Alternative Retirement Plan (ARP) with a 14–18% UC contribution.
Real Work‑Life Balance
– generous time‑off policy including vacation, sick time, 11 paid holidays, end‑of‑year paid time off, and six weeks paid parental leave.
Competitive salary
based on experience.
Comprehensive health coverage
(medical, dental, vision, prescription).
Flexible spending accounts & wellness programs .
Professional development & mentorship opportunities .
Equal Opportunity Employer
Building a workplace where all qualified applicants will receive consideration for employment, including Individuals with Disabilities and Protected Veterans.
#J-18808-Ljbffr