Research Assistant I - Laboratory of Dr. Joe Ecker
Salk Institute Office of Technology Development, San Diego, CA, United States
The Research Assistant I will work as part of a team with the goal of using epigenomics to create an atlas of the human brain at single-cell resolution, through both sequencing and imaging. This position will involve training in specialized techniques related to the Ecker lab focus of single cell multiomics. Main duties for this position may include large-scale generation of methylation sequencing libraries using a robotic pipetting system, Cryostat sectioning and fluorescence imaging for spatial transcriptomics through MERSCOPE, nuclei production and sorting, and treatment of nuclei to evaluate chromatin conformation. Other duties include general laboratory maintenance, coordination with collaborators and FACS core for cell sorting, participation in regular team meetings to discuss progress, and well-organized LIMS and data recording.
The Research Assistant I will work closely with lab staff and scientists in the laboratory to support ongoing research efforts.
This position requires strong experimental skills, including sterile technique, molecular cloning, DNA and RNA preparation, gel electrophoresis, genotyping, PCR, root sectioning and microscopy. It requires careful attention to detail and the ability to effectively balance research support responsibilities within a collaborative scientific environment.
What Your Key Responsibilities Will Be
Perform duties to support experiments on a variety of experiments, using bisulfite sequencing, magnetic bead DNA purification and PCR.
Perform tissue sectioning with Cryostat
Fluorescence staining protocols for imaging
Isolate cellular nuclei from brain tissue
Chromatin assays
Sample tracking and scheduling
Coordination with others for sample processing
Updating LIMS as necessary with sample information
Attend weekly lab meetings and assist with problem solving
General lab/equipment upkeep
Perform other related duties as assigned by management
What We Require
No professional experience required.
Bachelor's degree in Biological Sciences or related scientific discipline required. A minimum of 2 years of experience in a scientific role is required for consideration of a Bachelor's degree in a different discipline.
Computer skills required: Microsoft Office
What We Can Offer
The expected pay range for this position is $18.00-$21.00 hourly. Salk Institute provides pay ranges representing its good faith estimate of what the institute reasonably expects to pay for a position. The pay offered to a selected candidate will be determined based on factors such as (but not limited to) the scope and responsibilities of the position, the qualifications of the selected candidate, departmental budget availability, internal equity, geographic location, and external market pay for comparable jobs.
Benefits
Salk Institute offers competitive benefits, including medical, dental, vision, retirement, paid time off, tuition reimbursement, patient advocacy services, and transit/parking program.
Equal Employment Opportunity Statement
The Salk Institute for Biological Studies is an Equal Opportunity Employer and is committed to providing equal access to opportunities for students, employees, applicants for employment and other visitors. Salk has also adopted and maintains a policy to encourage professional and respectful workplace behavior and prevent discriminatory and harassing conduct in our workplace. Accordingly, the Institute prohibits harassment and discrimination in employment on the basis of, and considers all qualified applicants for employment without regard to, actual or perceived race (race is inclusive of traits associated with race, including, but not limited to, hair texture and protective hairstyles. Protective hairstyles include, but limited to, such hairstyles as braids, and twists), color, religion, religious creed (including religious dress and grooming practices), national origin, ancestry, citizenship, physical or mental disability, medical condition (including cancer and genetic characteristics), genetic information, marital status, age, sex (including pregnancy, childbirth, breastfeeding, or related medical conditions), reproductive health decision making, gender, gender identity, gender expression, sexual orientation, veteran and/or military status (disabled veteran, veteran of the Vietnam era, other covered veteran status), political affiliation, and any other status protected by state or federal law. Discrimination is prohibited with any intersectionality of the above-mentioned characteristics, including any combination of characteristics, a perception that the person has any of the characteristics or any combination of those characteristics, a perception that the person is associated with a person who has, or is perceived to have, any of those characteristics or any combination of those characteristics.
#J-18808-Ljbffr
The Research Assistant I will work closely with lab staff and scientists in the laboratory to support ongoing research efforts.
This position requires strong experimental skills, including sterile technique, molecular cloning, DNA and RNA preparation, gel electrophoresis, genotyping, PCR, root sectioning and microscopy. It requires careful attention to detail and the ability to effectively balance research support responsibilities within a collaborative scientific environment.
What Your Key Responsibilities Will Be
Perform duties to support experiments on a variety of experiments, using bisulfite sequencing, magnetic bead DNA purification and PCR.
Perform tissue sectioning with Cryostat
Fluorescence staining protocols for imaging
Isolate cellular nuclei from brain tissue
Chromatin assays
Sample tracking and scheduling
Coordination with others for sample processing
Updating LIMS as necessary with sample information
Attend weekly lab meetings and assist with problem solving
General lab/equipment upkeep
Perform other related duties as assigned by management
What We Require
No professional experience required.
Bachelor's degree in Biological Sciences or related scientific discipline required. A minimum of 2 years of experience in a scientific role is required for consideration of a Bachelor's degree in a different discipline.
Computer skills required: Microsoft Office
What We Can Offer
The expected pay range for this position is $18.00-$21.00 hourly. Salk Institute provides pay ranges representing its good faith estimate of what the institute reasonably expects to pay for a position. The pay offered to a selected candidate will be determined based on factors such as (but not limited to) the scope and responsibilities of the position, the qualifications of the selected candidate, departmental budget availability, internal equity, geographic location, and external market pay for comparable jobs.
Benefits
Salk Institute offers competitive benefits, including medical, dental, vision, retirement, paid time off, tuition reimbursement, patient advocacy services, and transit/parking program.
Equal Employment Opportunity Statement
The Salk Institute for Biological Studies is an Equal Opportunity Employer and is committed to providing equal access to opportunities for students, employees, applicants for employment and other visitors. Salk has also adopted and maintains a policy to encourage professional and respectful workplace behavior and prevent discriminatory and harassing conduct in our workplace. Accordingly, the Institute prohibits harassment and discrimination in employment on the basis of, and considers all qualified applicants for employment without regard to, actual or perceived race (race is inclusive of traits associated with race, including, but not limited to, hair texture and protective hairstyles. Protective hairstyles include, but limited to, such hairstyles as braids, and twists), color, religion, religious creed (including religious dress and grooming practices), national origin, ancestry, citizenship, physical or mental disability, medical condition (including cancer and genetic characteristics), genetic information, marital status, age, sex (including pregnancy, childbirth, breastfeeding, or related medical conditions), reproductive health decision making, gender, gender identity, gender expression, sexual orientation, veteran and/or military status (disabled veteran, veteran of the Vietnam era, other covered veteran status), political affiliation, and any other status protected by state or federal law. Discrimination is prohibited with any intersectionality of the above-mentioned characteristics, including any combination of characteristics, a perception that the person has any of the characteristics or any combination of those characteristics, a perception that the person is associated with a person who has, or is perceived to have, any of those characteristics or any combination of those characteristics.
#J-18808-Ljbffr